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KMID : 1161520000040040361
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Animal Cells and Systems
2000 Volume.4 No. 4 p.361 ~ p.366
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Protein kinase Ca is involved in the cell condensation during chondrogenesis in vitro
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Lim Young-Bin
Kang Shin-Sung
Lee Young-Sup
Sonn Jong-Kyung
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Abstract
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In order to investigate the role of protein kinase C (PKC) in chondrogenic differentiation, we examined the localization of PKC isoforms in a limb bud micromass culture system. PKCα is specifically localized in the regions which would become cartilage nodules, while PKCλ/ι and ζ display widespread distribution in the whole culture. Distribution of PKCa change along with promotion or inhibition of chondrogenesis by lysophosphatidylcholine or phorbol 12?myristate 13?acetate. On the other hand, localization of PKCA/i or ζ was not changed by the modulation of chondrogenesis. Peanut agglutinin binding protein which is associated with cell aggregation during chondrogenesis was present in the cell condensation regions and its expression in those regions was influenced by PKC activity. Expression of fibronectin and N?cadherin in the cell condensing area were also affected by modulation of PKC activity. These results suggest involvement of PKCα in the cell condensation, possibly through regulating expression of fibronectin and N?cadherin.
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KEYWORD
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Chondrogenesis, Cell condensation, Protein kinase C isoforms
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